LIFE SCIENCES, ANALYTICAL & MEDICAL TECHNOLOGIES

Equipment Applications for Hematology

Principle & OEM Spectroscopy Components

Hematology

Hematology relates to the study of the cause, prognosis, treatment, and prevention of diseases related to blood.

CO-Oximetry focuses on the measurement of oxygenation of blood. 

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CO-Oximetry and Spectroscopy methodology

CO-Oximetry relates to the measurement of the oxygen carrying state of hemoglobin, including oxygen-carrying hemoglobin (O2Hb), non-oxygen-carrying but normal hemoglobin (HHb) as well as carboxyhemoglobin (COHb) and methemoglobin (MetHb). A CO-Oximeter measures the absorption of VIS light passing through the blood, in order to distinguish oxyhemoglobin and deoxyhemoglobin and determine the oxyhemoglobin saturation.

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Spectral benches for Hematology & CO-Oximetry

Absorbance signals are weak. To maintain an adequate SNR and, more importantly, an excellent linearity, it is essential to select an optical spectrometer platform with very low stray light and high throughput. Concave gratings, by their design, correction of the aberrations and simplicity of use deliver the required ultimate performance. tec5USA CGS and MCS Series of spectrometers are the ideal candidates for optimized absorbance measurements in CO-oximeters.

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    Frequently Asked Questions at tec5USA

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    Typical applications include white light interference for thin film analysis, UV absorption of proteins for quantitative analysis, colorimetry, impurity detection in water, cleaning validation for API manufacturing, polymerization inhibitor monitoring, electroplating bath monitoring....

    The spectroscopic methodology is determined by which parameters are important to monitor during a process. For example, if you want to monitor protein concentration in a bioreactor, in which the biosynthesis takes place in an aqueous medium, then you likely would want to use Raman spectroscopy for the application, as water does not contribute to the Raman signal. Alternatively, if moisture content is important, water has very strong absorption in the NIR due to several vibrational and combination modes that can be monitored; water is transparent in the UV and visible spectral region. Understanding which chemical is important as there could be various factors that influence the choice of methodology....

    NIR spectroscopy is utilized across a variety of industries for qualitative and quantitative product analysis. Typical industries include Chemistry, Pharmacology, Food Feed & Beverage, Agriculture, and others. NIR spectroscopy is well suited for species containing C-H, N-H & O-H bonds, making it a wide-range technology for a variety of applications such as moisture, fat, oil, alcohol, APIs, polymers, etc....

    Raman spectroscopy is a technique which is used for several markets. These industries include Oil and Gas, Pharmacology, Biotechnology, Petrochemistry and many others. Due to the high selectivity of Raman spectroscopy, it is a powerful tool for many applications including, hydrocarbon analysis, bioreactor protein monitoring, crystallization monitoring, API concentration, polymer identification, surfactant analysis, natural gas components and several others....

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