LIFE SCIENCES, ANALYTICAL & MEDICAL TECHNOLOGIES

Equipment Applications for DNA Sequencing and PCR

Principle & OEM Spectroscopy Components

DNA Sequencing and PCR

DNA sequencing determines the order of nucleotides within a DNA molecule. This method reveals valuable information about the role of inheritance and susceptibility to disease. It is employed in medical diagnosis and treatment through gene therapy.

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Sanger Sequencing and Fluorescence Spectroscopy

Sanger sequencing is a DNA sequencing technique using the principle of Fluorescence spectroscopy. After the amplification step, PCR is halted by the addition of ddNTPs tagged with different fluorophores. The DNA fragments are arranged in size by capillary gel electrophoresis, passing in front a laser excitation. Specific fluorescence bands are emitted and the resulting chromatogram is processed for analysis.

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Spectral Benches for DNA Sequencing

Fluorescence signals are weak. To guarantee adequate SNR and LOD levels, it is critical to select an optical bench with high throughput. Concave grating spectrometers, by their design, deliver the required ultimate performance. When configured with high QE, low read noise back-thinned CCD sensors, cooled or uncooled, these spectrometers deliver the best system combination. tec5USA CGS and MCS Series of CCD spectrometers are the ideal candidates for high throughput DNA sequencing.

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    FAQ

    Frequently Asked Questions at tec5USA

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    Typical applications include white light interference for thin film analysis, UV absorption of proteins for quantitative analysis, colorimetry, impurity detection in water, cleaning validation for API manufacturing, polymerization inhibitor monitoring, electroplating bath monitoring....

    The spectroscopic methodology is determined by which parameters are important to monitor during a process. For example, if you want to monitor protein concentration in a bioreactor, in which the biosynthesis takes place in an aqueous medium, then you likely would want to use Raman spectroscopy for the application, as water does not contribute to the Raman signal. Alternatively, if moisture content is important, water has very strong absorption in the NIR due to several vibrational and combination modes that can be monitored; water is transparent in the UV and visible spectral region. Understanding which chemical is important as there could be various factors that influence the choice of methodology....

    NIR spectroscopy is utilized across a variety of industries for qualitative and quantitative product analysis. Typical industries include Chemistry, Pharmacology, Food Feed & Beverage, Agriculture, and others. NIR spectroscopy is well suited for species containing C-H, N-H & O-H bonds, making it a wide-range technology for a variety of applications such as moisture, fat, oil, alcohol, APIs, polymers, etc....

    Raman spectroscopy is a technique which is used for several markets. These industries include Oil and Gas, Pharmacology, Biotechnology, Petrochemistry and many others. Due to the high selectivity of Raman spectroscopy, it is a powerful tool for many applications including, hydrocarbon analysis, bioreactor protein monitoring, crystallization monitoring, API concentration, polymer identification, surfactant analysis, natural gas components and several others....

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