LIFE SCIENCES, ANALYTICAL & MEDICAL TECHNOLOGIES

Equipment Applications for Flow Cytometry

Principle & OEM Spectroscopy Components

Flow Cytometry

Extensive work and new developments in Immunology or Oncology require a thorough analysis and understanding of cell populations and characteristics. Flow cytometry is an essential tool to assist scientists in their research.

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What is Flow Cytometry?

Flow cytometry is an analytical technique for the sorting, counting, detection and classification of cells. Cells are tagged with specific fluorescent reagents (expression proteins, conjugated antibodies, binding dyes, etc…) and pass through a capillary in a sheath fluid onto which one, or more, excitation lasers are focused. The resulting Visible Emission Fluorescence is collected by a multichannel detection system consisting of highly sensitive sensors, collecting up to more than 30 detectable parameters.

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Spectral Engines for Flow Cytometry

Weak VIS Fluorescence signals, resulting from the excitation of one or multiple laser sources of different fluorescent reagents, are detected by highly sensitive sensors such as PMTs or APDs. While combinations of dichroic and bandpass filters have been the traditional layout of flow cytometers, new designs now incorporate spectrometers with linear arrays of PMTs or MPPCs in order to collect the complete spectral information. The design of these new “spectral” flow cytometers requires a complete understanding of the critical requirements at stake, such as throughput, stray light rejection and stability.

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    Typical applications include white light interference for thin film analysis, UV absorption of proteins for quantitative analysis, colorimetry, impurity detection in water, cleaning validation for API manufacturing, polymerization inhibitor monitoring, electroplating bath monitoring....

    The spectroscopic methodology is determined by which parameters are important to monitor during a process. For example, if you want to monitor protein concentration in a bioreactor, in which the biosynthesis takes place in an aqueous medium, then you likely would want to use Raman spectroscopy for the application, as water does not contribute to the Raman signal. Alternatively, if moisture content is important, water has very strong absorption in the NIR due to several vibrational and combination modes that can be monitored; water is transparent in the UV and visible spectral region. Understanding which chemical is important as there could be various factors that influence the choice of methodology....

    NIR spectroscopy is utilized across a variety of industries for qualitative and quantitative product analysis. Typical industries include Chemistry, Pharmacology, Food Feed & Beverage, Agriculture, and others. NIR spectroscopy is well suited for species containing C-H, N-H & O-H bonds, making it a wide-range technology for a variety of applications such as moisture, fat, oil, alcohol, APIs, polymers, etc....

    Raman spectroscopy is a technique which is used for several markets. These industries include Oil and Gas, Pharmacology, Biotechnology, Petrochemistry and many others. Due to the high selectivity of Raman spectroscopy, it is a powerful tool for many applications including, hydrocarbon analysis, bioreactor protein monitoring, crystallization monitoring, API concentration, polymer identification, surfactant analysis, natural gas components and several others....

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