Equipment Applications for Capillary Electrophoresis

Principle & OEM Spectroscopy Components

Capillary Electrophoresis is a technique to separate ions based on their electrophoretic mobility with an applied voltage. Mobility depends on the charge of the molecule, viscosity, friction and the atom’s radius. Speed is proportional to the applied field.

CE is an analytical method delivering fast results and high-resolution separation.

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Capillary Electrophoresis Principle of Operation

A CE system consists of a HV power supply, a sample introduction system, a capillary tube, a detector and an output device. Each side of the HV psu is connected to an electrode to initiate migration through the tube. The capillary is made of fused silica coated with polyimide. Each side of the capillary tube is dipped in a vial containing the electrode and a buffer. A small window near the end of the capillary allows light to pass through the analyte and run spectroscopic measurements.

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Spectroscopy for UV Absorbance, Label-Free, Capillary Electrophoresis

For assessing oligonucleotide purity, label-free UV Absorbance CE is the method of choice providing superior quantification, a non-toxic option and an improved quantification compared to fluorescent labelling. Advantages of UV Absorbance include high resolving power, direct on-line UV detection and simplified operation. Several PDA and multi-channel spectrometer platforms, such as tec5USA’s E-MUX MMS solutions, have been designed to answer the modular and multi-capillary requirements of modern CE instrumentation.

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    Frequently Asked Questions at tec5USA

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    Typical applications include white light interference for thin film analysis, UV absorption of proteins for quantitative analysis, colorimetry, impurity detection in water, cleaning validation for API manufacturing, polymerization inhibitor monitoring, electroplating bath monitoring....

    The spectroscopic methodology is determined by which parameters are important to monitor during a process. For example, if you want to monitor protein concentration in a bioreactor, in which the biosynthesis takes place in an aqueous medium, then you likely would want to use Raman spectroscopy for the application, as water does not contribute to the Raman signal. Alternatively, if moisture content is important, water has very strong absorption in the NIR due to several vibrational and combination modes that can be monitored; water is transparent in the UV and visible spectral region. Understanding which chemical is important as there could be various factors that influence the choice of methodology....

    NIR spectroscopy is utilized across a variety of industries for qualitative and quantitative product analysis. Typical industries include Chemistry, Pharmacology, Food Feed & Beverage, Agriculture, and others. NIR spectroscopy is well suited for species containing C-H, N-H & O-H bonds, making it a wide-range technology for a variety of applications such as moisture, fat, oil, alcohol, APIs, polymers, etc....

    Raman spectroscopy is a technique which is used for several markets. These industries include Oil and Gas, Pharmacology, Biotechnology, Petrochemistry and many others. Due to the high selectivity of Raman spectroscopy, it is a powerful tool for many applications including, hydrocarbon analysis, bioreactor protein monitoring, crystallization monitoring, API concentration, polymer identification, surfactant analysis, natural gas components and several others....


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