PHARMACOLOGY & BIOTECHNOLOGY

Equipment Applications for Upstream Processing

Upstream

In this area of bioprocessing, cells are grown in a bioreactor under controlled conditions to yield either a product or an organism which contains a product.  Upstream processing is related to all stages as cell isolation, cell cultivation, media preparation, cell banking & storage to culture expansion until harvest. 

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Bioreactors

Bioreactors vary in size and material of construction. The implementation is the same. They are used to grow biological samples in biopharmaceutical manufacturing.

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On-Line Analysis

There are many parameters that are monitored during a biological production run. Simple analysis such as dissolved O2 and pH keep the bacteria growing in a stable environment. In-situ spectroscopy techniques can be implemented to analyze different parameters such as media consumption, lactose and glucose levels throughout the process.

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Protein Titers

Spectroscopy can be used to measure protein concentrations in real time during runs. This enables proper manufacturing of the batch or trigger different alarms if the production run is trending outside of desired levels.

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    Typical applications include white light interference for thin film analysis, UV absorption of proteins for quantitative analysis, colorimetry, impurity detection in water, cleaning validation for API manufacturing, polymerization inhibitor monitoring, electroplating bath monitoring....

    The spectroscopic methodology is determined by which parameters are important to monitor during a process. For example, if you want to monitor protein concentration in a bioreactor, in which the biosynthesis takes place in an aqueous medium, then you likely would want to use Raman spectroscopy for the application, as water does not contribute to the Raman signal. Alternatively, if moisture content is important, water has very strong absorption in the NIR due to several vibrational and combination modes that can be monitored; water is transparent in the UV and visible spectral region. Understanding which chemical is important as there could be various factors that influence the choice of methodology....

    NIR spectroscopy is utilized across a variety of industries for qualitative and quantitative product analysis. Typical industries include Chemistry, Pharmacology, Food Feed & Beverage, Agriculture, and others. NIR spectroscopy is well suited for species containing C-H, N-H & O-H bonds, making it a wide-range technology for a variety of applications such as moisture, fat, oil, alcohol, APIs, polymers, etc....

    Raman spectroscopy is a technique which is used for several markets. These industries include Oil and Gas, Pharmacology, Biotechnology, Petrochemistry and many others. Due to the high selectivity of Raman spectroscopy, it is a powerful tool for many applications including, hydrocarbon analysis, bioreactor protein monitoring, crystallization monitoring, API concentration, polymer identification, surfactant analysis, natural gas components and several others....

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